?CHEMICAL INVESTIGATION AND ELICITOR ACTIVITY OF POLYSACCHARIDE OF RED ALGAE HYPNEA MUSCIFORMIS AND BOTRYOCLADIA LEPTOPODA
Keywords:
Seaweed,Elicitor,Phytoalexin,Browning.Abstract
Hypnea musciformis andBotryocladia leptopoda (red algae),collected fromKarachi coast were studied forchemical
investigation andelicitor activity.Ashcontent ofH.musciformis wasbit high(40%).Yieldsof HighMolecular Weight
Crude Elicitor Preparations HM.WCEP "Polysaccharides"of two algal genus were high (14-49%) in NaOH extracts.
These HMWCEP werechemically analysedfortotal sugarprotein,S04groupanduronicacidcontents. Simpleprofile
of monosaccharide consisting of galactose, glucose,fucose, mannose and galactoronic acid weredetected in acid and
aqueous extracts ofH. musciformis andB. leptopoda respectivelyascomparedto its alkaline extracts.Elicitor activity
of HMWCEP was determined in terms of induced browning in Cicer arietinum (chick pea) tissues. A pronounced
browning was produced by the samplestreated with various extracts of H. musciformis. The response was low in the
samples treated with various preparations of B. leptopoda.
